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One thing that I remember. I wonder if they have used any option to reinstall the game, because I have done that. Скачайте игра в самый начал и спокойно.The SV40 recombinants are useful for studies on the mechanism of DNA tumorigenesis (Kaneda et al., Virology 127, 1-13 (1986)). Thus, the recombinant DNA technique has a high practical value. To construct a recombinant virus efficiently, it is preferable to use a helper virus which is capable of suppressing the replication of the recombinant virus, and to obtain a stable cell line by the technique of Dr. Greenberg (J. Virol. 50, 715-726 (1986)). Among the currently available helper viruses, the helper virus designated as VHL (rat adenovirus type 3) is advantageous because of its wide host-range. Furthermore, the VHL can be easily obtained. However, the recombinant virus constructed by using VHL and SV40 lacks the ability to replicate and produce offspring in cultured cells.
On the other hand, some helper viruses known as “defective helper viruses” do not replicate their own genome due to mutations. The host-range of these helper viruses is narrow, and therefore, it is necessary to maintain the helper viruses in cell lines.
The present inventors thought that the co-existence of a helper virus and its recombinant virus in a cell line would be beneficial. Thus, the present invention was arrived at.{
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